ATP Citrate Lyase (ACLY) (AA 306-502) antibody

Details for Product No. ABIN1098143
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
ACLY, DDBDRAFT_0205389, DDBDRAFT_0235360, DDB_0205389, DDB_0235360, ACL, BcDNA:LD21334, CG8322, DmATPCL, Dmel\\CG8322, anon-WO0140519.179, dATPCL, l(2)01466, l(2)k09217, n(2)k09217, ATPCL, CLATP, A730 ... show more
ACLY, DDBDRAFT_0205389, DDBDRAFT_0235360, DDB_0205389, DDB_0235360, ACL, BcDNA:LD21334, CG8322, DmATPCL, Dmel\\CG8322, anon-WO0140519.179, dATPCL, l(2)01466, l(2)k09217, n(2)k09217, ATPCL, CLATP, A730098H14Rik, AW538652, Clatp, acly, cb722, zgc:92008 show less
Epitope
AA 306-502
(17), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
(70), (44), (43), (25), (25), (25), (24), (4), (3)
Host
Mouse
(62), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(55), (27), (20), (16), (9), (9), (8), (5), (3), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 3 to 4 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN1098143
396.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of human ACLY (AA 306-502 ) expressed in E. coli.
Clone 5F8D11
Isotype IgG1
Purification Purified
Alternative Name ACLY
Background Synonyms:
ACL, ATPCL, CLATP
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacηte from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. ,,,
Molecular Weight 125 kDa
Gene ID 47
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/50, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (2) Figure 4: Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple) .
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (3) Figure 3: Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (4) Figure 6: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining.
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (5) Figure 5: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining.
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (6) Figure 2: Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody (7) Figure 1: Western blot analysis using ACLY mAb against human ACLY recombinant protein. (Expected MW is 46.7 kDa)
Product cited in: Beckner, Fellows-Mayle, Zhang et al.: "Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 10, pp. 2282-95, 2010 (PubMed).

Chu, Lin, Hendel et al.: "ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 285, Issue 42, pp. 32606-15, 2010 (PubMed).

Validation Images
Did you look for something else?
back to top