CLCN3 antibody (AA 98-115)

Catalog No. ABIN1098197

Product Details anti-CLCN3 Antibody

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Binding Specificity: AA 98-115

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLCN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Purpose: Anti-Chloride Channel Protein 3 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Clcn3. It does not cross-react with Clcn4 or Clcn5.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): No cross Reactivity with Cav3.2

Purification: Purified by Protein G affinity chromatography.

Immunogen: Synthetic peptide correspond- ing to aa 98-115 (cytoplasmic N-terminus) of rat Clcn3 (accession no. NP_445815.2).

Clone: S258-5

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-2 μg/mL. A band of ~90 kDa is detected.
Immunohistochemistry: use at 1-10 μg/mL.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain membranes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for CLCN3

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Alternative Name: Chloride Channel Protein 3 (Clcn3) (CLCN3 Products)

Synonyms: CLC3 antibody, ClC-3 antibody, Clc3 antibody, CLCN3 antibody, fb78c02 antibody, wu:fb78c02 antibody, clc3 antibody, clc-3 antibody, chloride voltage-gated channel 3 antibody, chloride channel, voltage-sensitive 3 antibody, chloride channel 3 antibody, chloride channel protein 3 antibody, chloride channel, voltage-sensitive 3 S homeolog antibody, CLCN3 antibody, Clcn3 antibody, clcn3 antibody, PTRG_03131 antibody, BDBG_05668 antibody, MCYG_04420 antibody, clcn3.S antibody

Background: H(+)/Cl(-) exchange transporter 3,[Isoform 1]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (By similarity). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity). {UniProtKB:P51790, PubMed:10915634}., [Isoform 2]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C (PubMed:8155321). It could help neuronal cells to establish short-term memory (PubMed:8155321). {PubMed:10915634, PubMed:8155321}.,Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Clcn3 mediates the exchange of chloride ions against protons and functions as an antiporter, contributing to the acidification of the endosome and synaptic vesicle lumen. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.,[Isoform 1]: Lysosome membrane, Late endosome membrane, Cell membrane, Early endosome membrane,Chloride channel protein 3, ClC-3, Chloride transporter ClC-3

NCBI Accession: NP_445815

UniProt: P51792