ATP7B antibody (AA 3-21)

Catalog No. ABIN1098199

Product Details anti-ATP7B Antibody

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Binding Specificity: AA 3-21

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP7B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Purpose: Anti-Copper-Transporting ATPase2 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Copper-Transporting ATPase2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G affinity chromatography.

Immunogen: Synthetic peptide correspond- ing to aa 3-21 (cytoplasmic N-terminus) of human Copper-Transporting ATPase2. (accession no. NP_000044.2).

Clone: S62-29

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-2 μg/mL. A band of ~160 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain membranes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Target Details for ATP7B

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Alternative Name: Copper-Transporting ATPase2 (ATP7B Products)

Synonyms: PWD antibody, WC1 antibody, WD antibody, WND antibody, Atp7a antibody, tx antibody, Hts antibody, PINA antibody, Wd antibody, ATP7B antibody, ATPase copper transporting beta antibody, ATPase, Cu++ transporting, beta polypeptide antibody, ATPase, Cu++ transporting, beta polypeptide (Wilson disease) antibody, copper-transporting ATPase 2 antibody, ATP7B antibody, Atp7b antibody, atp7b antibody, LOC592143 antibody

Background: P-type Cu(+) transporter,The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from cells. ATP7b (also known as Copper-Transporting ATPase2) transports copper in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene: A is found in the liver, kidney and brain, the shorter form B is found in brain, and the third isoform, known as WND/140KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.

UniProt: B7ZLR4

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process