SLC1A1 antibody (AA 1-524)

Catalog No. ABIN1098200

Product Details anti-SLC1A1 Antibody

Target: SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))

Binding Specificity: AA 1-524

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Purpose: Anti-EAAC1 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human EAAC1.

Cross-Reactivity: Human

Purification: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein corresponding to aa 1-524 (full-length) of human EAAC1 (accession no. NP_004161.4).

Clone: S180-41

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL. A band of ~60 kDa is detected.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain membranes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Target Details for SLC1A1

Target: SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))

Alternative Name: EAAC1 (SLC1A1 Products)

Synonyms: GB16911 antibody, EAAT3 antibody, SLC1A2a antibody, zgc:91959 antibody, EAAC1 antibody, SCZD18 antibody, D130048G10Rik antibody, EAAC2 antibody, MEAAC1 antibody, Eaac1 antibody, Eaat3 antibody, REAAC1 antibody, excitatory amino acid transporter 3 antibody, solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody, solute carrier family 1 member 1 antibody, LOC412382 antibody, slc1a1 antibody, SLC1A1 antibody, CpipJ_CPIJ000674 antibody, CpipJ_CPIJ001134 antibody, Slc1a1 antibody

Background: Excitatory amino acid transporter 3,Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7914198, PubMed:7521911, PubMed:8857541, PubMed:26690923, PubMed:21123949). Can also transport L-cysteine (PubMed:21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:7521911, PubMed:8857541, PubMed:26690923). Mediates Cl(-) flux that is not coupled to amino acid transport, this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:8857541, PubMed:26690923). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity). {UniProtKB:P51906, UniProtKB:P51907, PubMed:21123949, PubMed:26690923, PubMed:7521911, PubMed:7914198, PubMed:8857541}.,Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent L- glutamate/D,L-aspartate cell membrane transport protein. It is expressed in brain as well as in several non-nervous system tissues. In brain, EAAC1 is the primary neuronal glutamate transporter. It has a polarized distribution in cells and functions mainly perisynaptically to transport glutamate from the extracellular environment. In the kidney it is involved in renal acidic amino acid reabsorption and amino acid metabolism. EAAC1- associated protein GTRAP3-18 may be important in regulating the metabolic function of EAAC1.,Cell membrane, Apical cell membrane, Cell junction, synapse, synaptosome, Early endosome membrane, Late endosome membrane, Recycling endosome membrane,Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1

UniProt: P43005

Pathways: Dicarboxylic Acid Transport