Phosphoglucomutase 1 antibody (Biotin)

Catalog No. ABIN1099701

Product Details anti-Phosphoglucomutase 1 Antibody (Biotin)

Target: Phosphoglucomutase 1 (PGM1)

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This Phosphoglucomutase 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Dot Blot (DB)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Physicochemical characteristics: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.8. No foreign proteins added. Marker N-Hydroxysuccinimidobiotin.

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Phosphoglucomutase isolated and purified from rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/20 and 1/500.

Comment:

Physical form: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.

Preservative: Without preservative

Handling Advice: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C

Target Details for Phosphoglucomutase 1

Target: Phosphoglucomutase 1 (PGM1)

Alternative Name: phosphoglucomutase (PGM1 Products)

Synonyms: CDG1T antibody, GSD14 antibody, ARABIDOPSIS THALIANA PHOSPHOGLUCOMUTASE antibody, ATPGMP antibody, MIO24.4 antibody, MIO24_4 antibody, PGM1 antibody, PHOSPHOGLUCOMUTASE antibody, STARCH-FREE 1 antibody, STF1 antibody, phosphoglucomutase antibody, PSPTO3035 antibody, CMS0426 antibody, 3230402E02Rik antibody, Pgm-1 antibody, Pgm2 antibody, zgc:63718 antibody, phosphoglucomutase-1 antibody, pgm2 antibody, PGM antibody, PGM 1 antibody, phosphoglucomutase 1 antibody, hypothetical protein antibody, phosphoglucomutase antibody, phosphoglucomutase alpha-D-glucose phosphate-specific Pgm antibody, phosphoglucomutase, alpha-D-glucose phosphate-specific antibody, phosphoglucomutase 1 L homeolog antibody, PGM1 antibody, R05F9.6 antibody, PGM antibody, pgm antibody, STY0736 antibody, PMI_RS02700 antibody, Pgm1 antibody, pgm1 antibody, LOC542721 antibody, pgm1.L antibody

Background: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Pathways: Cellular Glucan Metabolic Process