ATP2B1 antibody (AA 312-327)

Catalog No. ABIN1100135

Product Details anti-ATP2B1 Antibody

Target: ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))

Binding Specificity: AA 312-327

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Bat, Guinea Pig, Hamster, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP2B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human ATP2B1 / PMCA1. This antibody is expected to recognize both reported isoforms (NP_001001323.1, NP_001673.2).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Porcine, Guinea pig (100%) Turkey, Chicken, Platypus (94%) Opossum (88%) Zebra finch, Pufferfish, Zebrafish (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KQDGAIENRNKAKAQD from an internal region of human ATP2B1 / PMCA1 (NP_001001323.1, NP_001673.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Porcine, Guinea pig (100%), Turkey, Chicken, Platypus (94%), Opossum (88%), Zebra finch, Pufferfish, Zebrafish (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:64000), WB (0.03 - 0.1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 150kD band observed in lysates of cell line HeLa (calculated MW of 135kD according to NP_001673.2). Recommended concentration: 0.03-0.1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATP2B1

Target: ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))

Alternative Name: ATP2B1 / PMCA1 (ATP2B1 Products)

Background: Name/Gene ID: ATP2B1
Subfamily: ATPase - P type, type IIB
Family: Transporter

Synonyms: ATP2B1, PMCA1, PMCA1kb, Plasma membrane calcium pump

Gene ID: 490

NCBI Accession: NP_001001323, NP_001673

UniProt: P20020

Pathways: Ribonucleoside Biosynthetic Process