BRWD1 antibody (AA 2137-2151)

Catalog No. ABIN1100308

Product Details anti-BRWD1 Antibody

Target: BRWD1 (Bromodomain and WD Repeat Domain Containing 1 (BRWD1))

Binding Specificity: AA 2137-2151

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BRWD1 antibody is un-conjugated

Application: ELISA

Specificity: Human BRWD1 / WDR9. This antibody is expected to recognize reported isoforms A and B (NP_061836.2, NP_387505.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%) Baboon, Monkey, Marmoset (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NVKISETTGNSKFRP from an internal region (near C-terminus) of human BRWD1 / WDR9 (NP_061836.2, NP_387505.1, NP_001007247.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%), Baboon, Monkey, Marmoset (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 100kD band in Human Heart and Skeletal Muscle lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 263kD according to NP_061836.2. The 100kD band was successfully blocked by incubation with the immunizing peptide. We found identical results with an antibody generated to a different part of the protein (EB11490).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BRWD1

Target: BRWD1 (Bromodomain and WD Repeat Domain Containing 1 (BRWD1))

Alternative Name: WDR9 / BRWD1 (BRWD1 Products)

Synonyms: WDR9 antibody, BRWD1 antibody, C21orf107 antibody, N143 antibody, 5330419I02Rik antibody, D530019K20Rik antibody, G1-403-16 antibody, Wdr9 antibody, repro5 antibody, bromodomain and WD repeat domain containing 1 antibody, bromodomain and WD repeat-containing protein 1 antibody, BRWD1 antibody, LOC100226455 antibody, brwd1 antibody, Brwd1 antibody

Background: Name/Gene ID: BRWD1

Synonyms: BRWD1, C21orf107, N143, Transcriptional unit N143, WD repeat-containing protein 9, WD repeat domain 9, WDR9, WD repeat protein WDR9-form2

Gene ID: 54014

NCBI Accession: NP_061836, NP_387505, NP_001007247

UniProt: Q9NSI6