beta Defensin 1 antibody

Catalog No. ABIN1101108

Product Details anti-beta Defensin 1 Antibody

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Defensin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BD-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BD-1.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7480 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hBD-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BD-1 (LS-C104561) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-1. Western Blot: To detect hBD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Alternative Name: DEFB1 / Beta Defensin 1 (DEFB1 Products)

Synonyms: BD1 antibody, DEFB-1 antibody, DEFB101 antibody, HBD1 antibody, AW260221 antibody, BD-1 antibody, DEFB1-like antibody, RHBD-1 antibody, CBD1 antibody, PBD-1 antibody, DEFB1 antibody, BNBD-1 antibody, defensin beta 1 antibody, avian beta-defensin 1 antibody, defensin, beta 1 antibody, beta-defensin 1-like antibody, beta-defensin 1 antibody, DEFB1 antibody, Defb1 antibody, AvBD1 antibody, LOC100861170 antibody

Background: Name/Gene ID: DEFB1

Synonyms: DEFB1, BD1, Beta-defensin 1, BD-1, Beta Defensin 1, DEFB-1, DEFB101, Defensin, beta 1, Defensin beta, HBD-1, Beta-defensin-1, HBD1

Gene ID: 1672

UniProt: P60022