FGF2 antibody

Catalog No. ABIN1101523

Product Details anti-FGF2 Antibody

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Brand: IHC-plus™

Specificity: Human FGF-basic

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human FGF-basic.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (0.25 - 0.4 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7426 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (LS-C104313) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF2 / BFGF (FGF2 Products)

Synonyms: BFGF antibody, FGF-2 antibody, FGFB antibody, HBGF-2 antibody, Fgf-2 antibody, Fgfb antibody, bFGF antibody, fibroblast growth factor 2 antibody, fibroblast growth factor 2 (basic) antibody, FGF2 antibody, Fgf2 antibody, fgf2 antibody

Background: Name/Gene ID: FGF2
Family: HBGF

Synonyms: FGF2, Basic fibroblast growth factor, FGFB, Fibroblast growth factor 2, FGF-2, Prostatropin, BFGF, HBGF-2

Gene ID: 2247

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins