GEM antibody (AA 34-46)

Catalog No. ABIN1101725

Product Details anti-GEM Antibody

Target: GEM (GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))

Binding Specificity: AA 34-46

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This GEM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human GEM. Reported variants represent identical protein: NP_005252.1, NP_859053.1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Galago, Horse (92%) Rabbit, Porcine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QKEPHQYSHRNRH from an internal region of human GEM (NP_005252.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Horse (92%), Rabbit, Porcine (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: In transfected HEK293 transiently expressing Human recombinant GEM bands of approx. 35+45kD are observed. These bands are not observed in the transfected HEK293 with empty vector and the antibody does not cross-react to related protein such as Rem, Rem2L or Rad. Data provided by Prof. R Andres, Univ. Kentucky, USA. A similar banding pattern we observed in Human Spleen. The calculated molecular size is 33.9kD according to NP_005252.1. Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GEM

Target: GEM (GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))

Alternative Name: GEM / KIR (GEM Products)

Synonyms: KIR antibody, GEM antibody, DKFZp470D0612 antibody, LOC100221129 antibody, kir antibody, xgem antibody, zgc:153003 antibody, Gem antibody, AV020497 antibody, GTP binding protein overexpressed in skeletal muscle antibody, geminin, DNA replication inhibitor antibody, GTP binding protein (gene overexpressed in skeletal muscle) antibody, GEM antibody, gem antibody, Gem antibody, GMNN antibody

Background: Name/Gene ID: GEM
Family: Ras GTPase superfamily IPR001806

Synonyms: GEM, GTP-binding protein GEM, KIR, RAS-like protein KIR

Gene ID: 2669

NCBI Accession: NP_005252

UniProt: P55040