NYS48/HAUS8 antibody (pSer70)

Catalog No. ABIN1101969

Product Details anti-NYS48/HAUS8 Antibody

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Binding Specificity: pSer70

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NYS48/HAUS8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100 % sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Purification: Protein A purified

Immunogen: Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1.22 μg/mL)

Usage: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kD in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for NYS48/HAUS8

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Alternative Name: HAUS8 (HAUS8 Products)

Synonyms: 2410004L22Rik antibody, Hice1 antibody, NY-SAR-48 antibody, hice1 antibody, DGT4 antibody, HICE1 antibody, Ny-sar-48 antibody, RGD1311565 antibody, 4HAUS augmin-like complex, subunit 8 antibody, HAUS augmin like complex subunit 8 L homeolog antibody, HAUS augmin like complex subunit 8 antibody, HAUS augmin-like complex, subunit 8 antibody, Haus8 antibody, haus8.L antibody, HAUS8 antibody

Background: Name/Gene ID: HAUS8

Synonyms: HAUS8, DGT4, NY-SAR-48, Sarcoma antigen NY-SAR-48, HICE1

Gene ID: 93323

UniProt: Q9BT25