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His Tag antibody (Biotin)

WB, ELISA, IHC Host: Mouse Monoclonal 33D10-D2 Biotin
Catalog No. ABIN1102040
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
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    Host
    • 93
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    Mouse
    Clonality
    • 106
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    Monoclonal
    Conjugate
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    This His Tag antibody is conjugated to Biotin
    Application
    • 153
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Sequence
    HHHHHH
    Specificity
    This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
    Purification
    Protein A purified
    Immunogen
    This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

    Type of Immunogen: Hapten
    Clone
    33D10-D2
    Isotype
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

    Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    deionized water
    Concentration
    Lot specific
    Buffer
    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    avoid freeze thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Target
    His Tag
    Abstract
    His Tag Products
    Target Type
    Tag
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