His Tag antibody (HRP)

Details for Product No. ABIN1102042, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
620
18
14
3
1
Host
Rabbit
413
180
27
23
4
4
3
1
Clonality
Polyclonal
Conjugate
HRP
58
45
36
15
13
11
10
9
9
8
8
8
8
8
8
7
7
7
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
545
385
164
106
77
75
68
26
12
11
9
7
7
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Type of Immunogen: Purified protein
Sequence HHHHHH
Isotype IgG
Specificity This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Purification Affinity purified
Target Type Tag
Research Area Tags/Labels
Application Notes Approved: ELISA, IHC (1:500 - 1:2000), WB (1:2000 - 1:5000)

Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only
Format Liquid
Reconstitution deionized water
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Supplier Images
 image for anti-His Tag antibody (HRP) (ABIN1102042) anti-His Tag antibody (HRP)
Did you look for something else?