His Tag antibody

Details for Product No. ABIN1102044, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
620
18
14
3
1
Host
Mouse
412
181
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
59
45
36
15
13
11
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8
8
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8
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4
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3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
545
385
164
106
77
76
68
26
12
11
9
7
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6
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4
4
3
2
2
1
1
1
1
1
1
1
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1
1
Options
Supplier
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Immunogen HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Type of Immunogen: Hapten
Sequence HHHHHH
Clone 33D10-D2-G8
Isotype IgG1
Specificity 6X HIS Epitope Tag antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Purification Purified
Sterility Sterile filtered
Target Type Tag
Research Area Tags/Labels
Application Notes Approved: ELISA, WB (1:1000)

Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
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