IL1RN antibody

Catalog No. ABIN1102536

Product Details anti-IL1RN Antibody

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Brand: IHC-plus™

Specificity: Human IL-1RA

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-1RA.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (2.5 - 3.7 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7435 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-1RA (50 ng/mL) and Human IL-1alpha (50 pg/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required. ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (LS-C104474) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL1RN (IL1RN Products)

Synonyms: DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MVCD4 antibody, IRAP1 antibody, IL1RN antibody, IL-1RA antibody, F630041P17Rik antibody, interleukin 1 receptor antagonist antibody, interleukin 1 receptor antagonist-like antibody, IL1RN antibody, Il1rn antibody, IL1RNL antibody

Background: Name/Gene ID: IL1RN
Family: IL-1

Synonyms: IL1RN, Anakinra, DIRA, IL-1ra, IL-1RN, IL1F3, IL1RA, MVCD4, IL1 inhibitor, IL1RN (IL1F3), IRAP, ICIL-1RA, IL-1ra3

Gene ID: 3557

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints