LYZ antibody (AA 1-129)

Catalog No. ABIN1102920

Product Details anti-LYZ Antibody

Target: LYZ (Lysozyme (LYZ))

Binding Specificity: AA 1-129

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White). A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100 % sequence homology. Lysozyme from quail and pheasant are also reported to be 100 % identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.

Purification: Ion exchange chromatography

Sterility: Sterile filtered

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC (1:500 - 1:2000), IP (1:100), WB (1:500 - 1:2000)

Usage: This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~14 kD in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: LYZ / Lysozyme (LYZ Products)

Synonyms: LZM antibody, 1 antibody, LYZC antibody, lys-C antibody, zgc:136734 antibody, BmLys antibody, LYS antibody, LZ antibody, LYZS antibody, lysozyme C antibody, LYZ antibody, lysozyme antibody, lysozyme antibody, lysozyme (renal amyloidosis) antibody, lysozyme L homeolog antibody, serum lysozyme antibody, LYZ antibody, lyz antibody, Lzm antibody, LOC781146 antibody, lyz.L antibody, S-LZ antibody

Background: Name/Gene ID: LYZ

Synonyms: LYZ, 1,4-beta-N-acetylmuramidase C, LZM, Lysozyme, Lysozyme (renal amyloidosis), Renal amyloidosis, Lysozyme C

Gene ID: 4069

UniProt: P61626