Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Brand
IHC-plus™
Specificity
Human LYZ / Lysozyme. The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity The antiserum does not cross-react with any other component of human plasma. Inter-species cross- reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Usage: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Sterile distilled water 1 ml
Concentration
Lot specific
Buffer
Lyophilized from PBS, pH 7.2
Handling Advice
Avoid freeze-thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.