MTHFR antibody (AA 369-383)

Catalog No. ABIN1103105

Product Details anti-MTHFR Antibody

Target: MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

Binding Specificity: AA 369-383

Reactivity: Human, Monkey, Dog, Cow, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This MTHFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human MTHFR.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Rabbit, Porcine, Medaka (100%) Mouse, Rat, Hamster, Bat, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus (93%) Guinea pig, Stickleback, Pufferfish, Zebrafish (87%) Seq squirt, Sea anemone, Water flea (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SRPKSYIYRTQEWDE from an internal region of human MTHFR (NP_005948.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Rabbit, Porcine, Medaka (100%), Mouse, Rat, Hamster, Bat, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus (93%), Guinea pig, Stickleback, Pufferfish, Zebrafish (87%), Seq squirt, Sea anemone, Water flea (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:8000), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 70kD band observed in lysates of cell line NIH3T3 (calculated MW of 74.6kD according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MTHFR

Target: MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

Alternative Name: MTHFR (MTHFR Products)

Synonyms: DDBDRAFT_0204686 antibody, DDBDRAFT_0230137 antibody, DDB_0204686 antibody, DDB_0230137 antibody, AI323986 antibody, methylenetetrahydrofolate reductase (NAD(P)H) L homeolog antibody, methylenetetrahydrofolate reductase antibody, methylenetetrahydrofolate reductase (NAD(P)H) antibody, 5,10-methylenetetrahydrofolate reductase antibody, mthfr.L antibody, MTHFR antibody, mthfr antibody, metF antibody, DDB_G0279137 antibody, Mthfr antibody

Background: Name/Gene ID: MTHFR

Synonyms: MTHFR

Gene ID: 4524

NCBI Accession: NP_005948

UniProt: P42898

Pathways: Methionine Biosynthetic Process