Myc Tag (AA 410-419) antibody

Details for Product No. ABIN1103152, Supplier: Log in to see
Antigen
Epitope
AA 410-419
55
10
6
4
3
2
2
2
2
2
1
1
Reactivity
Tag
212
36
14
10
6
5
3
1
1
Host
Rabbit
161
59
17
16
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
27
23
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
215
115
78
53
47
34
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.

Type of Immunogen: Purified protein
Isotype IgG
Specificity This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Purification Affinity purified
Sterility Sterile filtered
Target Type Tag
Research Area Tags/Labels
Application Notes Approved: ELISA, IHC, WB (1:500 - 1:5000)

Usage: Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Supplier Images
 image for anti-Myc Tag (AA 410-419) antibody (ABIN1103152) anti-Myc Tag (AA 410-419) antibody
 image for anti-Myc Tag (AA 410-419) antibody (ABIN1103152) anti-Myc Tag (AA 410-419) antibody (Image 2)