Neuregulin 1 antibody

Catalog No. ABIN1103330

Product Details anti-Neuregulin 1 Antibody

Target: Neuregulin 1 (NRG1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuregulin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Brand: IHC-plus™

Specificity: Human Heregulin-beta1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Heregulin-beta1.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut, WB

Usage: Immunohistochemistry: LS-B7486 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human Heregulin-beta1 (1 ng/mL), a concentration of 0.7-1.1 μg/mL of this antibody is required. ELISA: To detect Human Heregulin-beta1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Heregulin-beta1(LS-C104552) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Heregulin-beta1. Western Blot: To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Neuregulin 1

Target: Neuregulin 1 (NRG1)

Alternative Name: NRG1 / Heregulin / Neuregulin (NRG1 Products)

Synonyms: 6030402G23Rik antibody, ARIA antibody, D230005F13Rik antibody, GGF antibody, GGFII antibody, HRG antibody, HRGalpha antibody, Hgl antibody, NDF antibody, SMDF antibody, GGF2 antibody, HGL antibody, HRG1 antibody, HRGA antibody, MST131 antibody, NRG-1 antibody, heregulin antibody, nrg1-A antibody, pro-neuregulin-1 antibody, NRG1 antibody, wu:fm70e01 antibody, DKFZp459A1838 antibody, neuregulin 1 antibody, histidine rich glycoprotein antibody, neuregulin 1 L homeolog antibody, Nrg1 antibody, NRG1 antibody, HRG antibody, nrg1.L antibody, nrg1 antibody

Background: Name/Gene ID: NRG1

Synonyms: NRG1, ARIA, GGF, GGF2, Glial growth factor, Heregulin, HRGA, MSTP131, NDF, Neu differentiation factor, Neuregulin 1 type IV beta 3, Neuregulin 1 type IV beta 1a, HRG1, NRG-1, SMDF, Neuregulin 1, Pro-NRG1, HGL, MST131

Gene ID: 3084

UniProt: Q02297

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation