RFP antibody (Red Fluorescent Protein) (Biotin)

Details for Product anti-RFP Antibody No. ABIN1103973, Supplier: Log in to see
Antigen
Reactivity
Discosoma
82
23
21
10
7
2
1
1
Host
Rabbit
69
37
14
9
1
Clonality
Polyclonal
Conjugate
This RFP antibody is conjugated to Biotin
6
5
5
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
106
70
47
39
31
29
7
5
4
3
2
1
1
1
1
1
1
Options
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Immunogen The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Type of Immunogen: Fusion protein
Isotype IgG
Specificity Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1 % of target signal.
Purification Affinity purified
Alternative Name RFP
Research Area Tags/Labels
Application Notes Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only
Format Liquid
Reconstitution deionized water
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
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 image for anti-RFP antibody (Red Fluorescent Protein)  (Biotin) (ABIN1103973) anti-Red Fluorescent Protein (RFP) antibody (Biotin)