SCP2 antibody (AA 163-175)

Catalog No. ABIN1104148

Product Details anti-SCP2 Antibody

Target: SCP2 (Sterol Carrier Protein 2 (SCP2))

Binding Specificity: AA 163-175

Reactivity: Human, Rat, Cow, Horse, Hamster, Monkey, Bat, Drosophila melanogaster, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This SCP2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SCP2. This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2, NP_001007099.1, NP_001180529.1, NP_001180528.1, NP_001180546.1 respectively),

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Drosophila, Ant, Beetle (100%) M. tuberculosis (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KNHKHSVNNPYSQ from an internal region of human SCP2 (NP_001007099.1, NP_002970.2, NP_001180528.1, NP_001180529.1, NP_001180546.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Drosophila, Ant, Beetle (100%), M. tuberculosis (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 28kD band in Human Adipose tissue lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0kD according to NP_001007099.1 and of 6.7kD according to NP_002970.2. The 28kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SCP2

Target: SCP2 (Sterol Carrier Protein 2 (SCP2))

Alternative Name: SCP2 / SCPX (SCP2 Products)

Synonyms: NLTP antibody, NSL-TP antibody, SCP-2 antibody, SCP-CHI antibody, SCP-X antibody, SCPX antibody, AA409774 antibody, AA409893 antibody, C76618 antibody, C79031 antibody, ns-LTP antibody, NSLIPTR antibody, SCPx antibody, MGC77634 antibody, zgc:77634 antibody, SCP2 antibody, MGC108409 antibody, ATSCP2 antibody, MBD2.8 antibody, MBD2_8 antibody, STEROL CARRIER PROTEIN 2 antibody, PLTP antibody, zgc:101621 antibody, sterol carrier protein 2 antibody, sterol carrier protein 2, liver antibody, sterol carrier protein 2a antibody, sterol carrier protein 2 L homeolog antibody, phospholipid transfer protein homolog1 antibody, uncharacterized LOC101116445 antibody, sterol carrier protein 2b antibody, SCP2 antibody, Scp2 antibody, scp2a antibody, scp2.L antibody, scp2 antibody, CC1G_13192 antibody, plt1 antibody, LOC101116445 antibody, scp2b antibody

Background: Name/Gene ID: SCP2

Synonyms: SCP2, NLTP, NSL-TP, SCP-CHI, SCP-X, Sterol carrier protein 2, Sterol carrier protein X, SCP-2, SCPX

Gene ID: 6342

NCBI Accession: NP_001007099, NP_002970, NP_001180528, NP_001180529, NP_001180546

UniProt: P22307

Pathways: C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process