V5 Epitope Tag (AA 95-108) antibody

Details for Product No. ABIN1104955, Supplier: Log in to see
Antigen
Epitope
AA 95-108
14
Reactivity
Tag
404
3
3
3
3
Host
Rabbit
221
134
37
9
5
4
3
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
29
27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
342
265
123
92
71
65
52
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.

Type of Immunogen: Synthetic peptide
Sequence GKPIPNPLLG LDST
Isotype IgG
Specificity This affinity-purified antibody is directed against V5 motif and is useful in determining its presence in various assays. This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivity with recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.
Purification Affinity purified
Sterility Sterile filtered
Alternative Name V5 Tag
Target Type Tag
Research Area Tags/Labels
Application Notes Approved: ELISA, IHC (1:500 - 1:3000), WB (1:5000 - 1:10000)

Usage: Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Aliquot to Avoid freeze/thaw cycles. Store undiluted.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
Supplier Images
 image for anti-V5 Epitope Tag (AA 95-108) antibody (ABIN1104955) anti-V5 Epitope Tag (AA 95-108) antibody