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His Tag antibody

WB, IP, IF Host: Mouse Monoclonal HIS-H8 unconjugated
Catalog No. ABIN1105141
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
    • 93
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    Mouse
    Clonality
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    Monoclonal
    Conjugate
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    This His Tag antibody is un-conjugated
    Application
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    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    Sequence
    HHHHHH
    Purification
    Affinity Chromatography.
    Immunogen
    Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
    Clone
    HIS-H8
    Isotype
    IgG2b
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    His Tag
    Alternative Name
    6xHistidine Epitope Tag (His Tag Products)
    Target Type
    Tag
    Background
    Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
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