His Tag antibody

Details for Product No. ABIN1105141, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
18
3
1
Host
Mouse
397
178
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
58
44
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
528
372
159
105
77
75
66
26
12
11
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
Sequence HHHHHH
Clone HIS-H8
Isotype IgG2b
Purification Affinity Chromatography.
Alternative Name 6xHistidine Epitope Tag
Target Type Tag
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN1105141) anti-His Tag antibody
Immunofluorescence (IF) image for anti-His Tag antibody (ABIN1105141) anti-His Tag antibody (Image 2)
Background publications Xu, Liu, He: "Expression and purification of soluble human programmed death-1 in Escherichia coli." in: Cellular & molecular immunology, Vol. 3, Issue 2, pp. 139-43, 2006 (PubMed).

Terpe: "Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems." in: Applied microbiology and biotechnology, Vol. 60, Issue 5, pp. 523-33, 2003 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).