His Tag antibody

Details for Product No. ABIN1105145, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
18
3
1
Host
Mouse
397
178
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
58
44
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
528
372
160
106
77
75
66
26
12
11
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen Synthetic peptide 6 His
Sequence HHHHHH
Clone 6C4
Isotype IgG1
Purification Protein A agarose
Alternative Name 6xHistidine Epitope Tag
Target Type Tag
Background A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The His-Tag fusion protein expression system is commonly used because the 6 x His-Tag on the recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer PBS containing 50 % glycerol, pH 7.2, without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN1105145) anti-His Tag antibody
Background publications Porath: "Immobilized metal ion affinity chromatography." in: Protein expression and purification, Vol. 3, Issue 4, pp. 263-81, 1992 (PubMed).