AKAP12 antibody (C-Term)

Catalog No. ABIN1105270

Product Details anti-AKAP12 Antibody

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This anrtibody is expected to recognize both reported isoforms of AKAP12.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence C-KQERESAKSELTES, from the C-Terminus of the protein sequence according to NP_005091.2, NP_653080.1. Genename: AKAP12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: AKAP12 (AKAP12 Products)

Synonyms: akap12 antibody, cb27 antibody, esr1 antibody, fb72g12 antibody, id:ibd1202 antibody, sb:cb27 antibody, si:ch73-192g21.1 antibody, wu:fb72g12 antibody, wu:fc21c03 antibody, AKAP12 antibody, AI317366 antibody, SSeCKS antibody, Srcs5 antibody, Tsga12 antibody, AKAP12A antibody, AKAP12B antibody, AKAP12G antibody, AKAP250 antibody, A kinase (PRKA) anchor protein 12b antibody, A-kinase anchoring protein 12 antibody, A kinase (PRKA) anchor protein (gravin) 12 antibody, akap12b antibody, AKAP12 antibody, Akap12 antibody

Background: Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.Synonyms: A-kinase anchor protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen, PRKA12

Molecular Weight: 191 kDa (NP_005091.2) and 181 kDa (NP_653080.1).

Gene ID: 9590

NCBI Accession: NP_005091

Pathways: cAMP Metabolic Process