SLPI antibody

Catalog No. ABIN1105383

Product Details anti-SLPI Antibody

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLPI antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein G

Immunogen: SLPI purified from sputum

Clone: 31

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for SLPI

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Alternative Name: Antileukoproteinase (ALP) (SLPI Products)

Synonyms: ALK1 antibody, ALP antibody, BLPI antibody, HUSI antibody, HUSI-I antibody, MPI antibody, WAP4 antibody, WFDC4 antibody, MATN4 antibody, SLPI antibody, ckpi antibody, secretory leukocyte peptidase inhibitor antibody, antileukoproteinase antibody, SLPI antibody, Slpi antibody, LOC102174044 antibody

Background: SLPI was identified as an alarm reactant and expression is induced by inflammatory factors like LPS, IL1β, TNFα and neutrophils elastase. SLPI is a relatively small basic antiprotease of 11.7 kDa and is a cationic non-glycosylated protein consisting of 107amino acids. SLPI has a high affinity for the neutrophil serine proteinases, elastase and cathepsin G. Orthologues of SLPI have been found in mice, rate, pigs and sheep. It consists of two highly similar WAP ('whey acid protein')/four-disulphide core domains. SLPI contain 16 cysteine residues which assemble into eight disulphide bridges (four in each WAP domain). SLPI is constitutively expressed at many mucosal surfaces and is produced by a variety of epithelial cells, including respiratory, intestinal and amniotic epithelia. Expression is also detected in mast cells, neutrophils and macrophages. Expression of SLPI gene is significantly increased by progesterone and by the pro-inflammatory cytokines TNF-α and IL1-β. Although SLPI has been shown to inhibit a spectrum of proteases (including HNE, cathepsin G, trypsin, chymotrypsin and chymase), its main action in this regard is likely to be the inhibition of elastase, as indicated by its low dissociation constant and favourable kinetics of inhibition for this enzyme. SLPI has been described in several body fluids like seminal fluid, bronchial fluids, cervical fluids and saliva. It has been found to be antibacterial, antifungal, anti-retroviral, and to have an important role in mucosal defence. SLPI might also facilitate tumor spread, contributing to wound healing, is elevated in sepsis and levels seem to correlate with oral candidiasis in HIV-1 positive patients.Synonyms: BLPI, HUSI-1, Mucus proteinase inhibitor, Protease inhibitor WAP4, SLPI, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four-disulfide core domain protein 4, WAP4, WFDC4

UniProt: P03973