AOC3 antibody

Catalog No. ABIN1105387

Product Details anti-AOC3 Antibody

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This AOC3 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: The monoclonal antibody 7-88 recognizes mouse Vascular Adhesion Protein-1 (VAP-1) which is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It inhibits migration of granulocytes and monocytes in acute models of inflammation.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein G Chromatography

Immunogen: Vessels from mouse lymph nodes

Clone: 01-07-88

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for AOC3

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Alternative Name: AOC3 / VAP1 (AOC3 Products)

Background: VAP-1 is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 is expressed in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity. In white adipose tissue of obese and diabetic db-/- mice increased expression of VAP-1 has been observed suggesting that it contributes to the arthrosclerosis and vascular dysfunction observed in these diseases. Moreover, inhibition of VAP-1reduced the accumulation of myeloid cells into tumors and attenuates tumor growth.Synonyms: Copper amine oxidase, HPAO, Membrane primary amine oxidase, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1

Gene ID: 11754

NCBI Accession: NP_033805

UniProt: O70423

Pathways: Feeding Behaviour