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B-Raf Proto-Oncogene antibody

Details for Product No. ABIN1105488, Supplier: Log in to see
Antigen
Reactivity
Human
16
6
4
1
1
Host
Mouse
14
1
1
Clonality (Clone)
Monoclonal ()
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
12
9
8
2
Supplier
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Clone 1H12F1
Isotype IgG1
Specificity Recognizes BRAF (V-raf murine sarcoma viral oncogene homolog B1).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ascites
Background BRAF (V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1
Gene ID 673
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 0.03 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
Western Blotting (WB) image for anti-B-Raf Proto-Oncogene antibody (ABIN1105488) anti-B-Raf Proto-Oncogene antibody
Western Blotting (WB) image for anti-B-Raf Proto-Oncogene antibody (ABIN1105488) anti-B-Raf Proto-Oncogene antibody (Image 2)
Immunohistochemistry (IHC) image for anti-B-Raf Proto-Oncogene antibody (ABIN1105488) anti-B-Raf Proto-Oncogene antibody (Image 3)
Immunohistochemistry (IHC) image for anti-B-Raf Proto-Oncogene antibody (ABIN1105488) anti-B-Raf Proto-Oncogene antibody (Image 4)
Background publications Kim, Giuliano, Turner et al.: "Lymphatic mapping establishes the role of BRAF gene mutation in papillary thyroid carcinoma." in: Annals of surgery, Vol. 244, Issue 5, pp. 799-804, 2006 (PubMed).

Noda, Kato, Yoshikawa et al.: "Frequent involvement of ras-signalling pathways in both polypoid-type and flat-type early-stage colorectal cancers." in: Journal of experimental & clinical cancer research : CR, Vol. 25, Issue 2, pp. 235-42, 2006 (PubMed).

Terai, Matsuda: "The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf." in: The EMBO journal, Vol. 25, Issue 15, pp. 3556-64, 2006 (PubMed).

Fullen, Poynter, Lowe et al.: "BRAF and NRAS mutations in spitzoid melanocytic lesions." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 19, Issue 10, pp. 1324-32, 2006 (PubMed).

Rapp, Goldsborough, Mark et al.: "Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 14, pp. 4218-22, 1983 (PubMed).