BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1)

Details for Product anti-BRAF Antibody No. ABIN1105488, Supplier: Log in to see
Antigen
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Alternatives
anti-Human BRAF antibody for Western Blotting
Reactivity
Human
406
85
69
13
10
4
4
3
1
1
1
1
Host
Mouse
309
93
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
267
210
145
78
70
45
39
22
10
8
5
4
1
1
1
1
Options
Supplier
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Supplier Product No.
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Clone 1H12F1
Isotype IgG1
Specificity Recognizes BRAF (V-raf murine sarcoma viral oncogene homolog B1).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ascites
Background BRAF (V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1
Gene ID 673
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 0.03 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 4)
Background publications Kim, Giuliano, Turner, Gaffney, Umetani, Kitago, Elashoff, Hoon: "Lymphatic mapping establishes the role of BRAF gene mutation in papillary thyroid carcinoma." in: Annals of surgery, Vol. 244, Issue 5, pp. 799-804, 2006 (PubMed).

Fullen, Poynter, Lowe, Su, Elder, Nair, Johnson, Gruber: "BRAF and NRAS mutations in spitzoid melanocytic lesions." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 19, Issue 10, pp. 1324-32, 2006 (PubMed).

Noda, Kato, Yoshikawa, Arai, Togashi, Nagai, Konishi, Miki: "Frequent involvement of ras-signalling pathways in both polypoid-type and flat-type early-stage colorectal cancers." in: Journal of experimental & clinical cancer research : CR, Vol. 25, Issue 2, pp. 235-42, 2006 (PubMed).

Terai, Matsuda: "The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf." in: The EMBO journal, Vol. 25, Issue 15, pp. 3556-64, 2006 (PubMed).

Rapp, Goldsborough, Mark, Bonner, Groffen, Reynolds, Stephenson: "Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 14, pp. 4218-22, 1983 (PubMed).

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