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BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1)

Details for Product anti-BRAF Antibody No. ABIN1105488, Supplier: Log in to see
Antigen
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Alternatives
anti-Human BRAF antibody for Western Blotting
Reactivity
Human
390
80
64
13
10
4
4
3
1
1
1
1
Host
Mouse
295
91
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
255
203
141
78
61
42
39
22
10
8
4
4
1
1
1
1
Options
Supplier
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Clone 1H12F1
Isotype IgG1
Specificity Recognizes BRAF (V-raf murine sarcoma viral oncogene homolog B1).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ascites
Background BRAF (V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1
Gene ID 673
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 0.03 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1105488) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 4)
Background publications Kim, Giuliano, Turner, Gaffney, Umetani, Kitago, Elashoff, Hoon: "Lymphatic mapping establishes the role of BRAF gene mutation in papillary thyroid carcinoma." in: Annals of surgery, Vol. 244, Issue 5, pp. 799-804, 2006 (PubMed).

Fullen, Poynter, Lowe, Su, Elder, Nair, Johnson, Gruber: "BRAF and NRAS mutations in spitzoid melanocytic lesions." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 19, Issue 10, pp. 1324-32, 2006 (PubMed).

Noda, Kato, Yoshikawa, Arai, Togashi, Nagai, Konishi, Miki: "Frequent involvement of ras-signalling pathways in both polypoid-type and flat-type early-stage colorectal cancers." in: Journal of experimental & clinical cancer research : CR, Vol. 25, Issue 2, pp. 235-42, 2006 (PubMed).

Terai, Matsuda: "The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf." in: The EMBO journal, Vol. 25, Issue 15, pp. 3556-64, 2006 (PubMed).

Rapp, Goldsborough, Mark, Bonner, Groffen, Reynolds, Stephenson: "Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 14, pp. 4218-22, 1983 (PubMed).