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Myc Tag antibody

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Antigen
Reactivity
Tag
199
26
3
2
1
1
1
Host
Mouse
118
75
14
14
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
24
17
11
8
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
179
84
66
51
39
29
11
11
8
7
6
5
5
4
4
4
4
2
1
1
1
1
1
1
1
1
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Immunogen GST-6myc-Tag fusion protein
Sequence EQKLISEEDL
Clone PL14
Isotype IgG1
Specificity This antibody can be used for epitope-tagging using the amino acid sequence EQKLISEEDL.
Purification Protein-A Sepharose
Alternative Name C-Myc Epitope Tag
Target Type Tag
Background Epitope tagging is a widely accepted technique that fuses an epitope peptide to a protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follows, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
Gene ID 4609
NCBI Accession NP_002458
UniProt P01106
Research Area Transcription Factors, Cancer, Cell Cycle
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer PBS containing 50 % glycerol, without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Heald, McLoughlin, McKeon: "Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase." in: Cell, Vol. 74, Issue 3, pp. 463-74, 1993 (PubMed).

Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).