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Myc Tag antibody (Biotin)

Details for Product No. ABIN1105585, Supplier: Log in to see
Antigen
Reactivity
Tag
200
26
3
2
1
1
1
Host
Mouse
119
75
14
14
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
24
17
11
8
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
180
84
67
51
40
29
11
11
8
7
6
5
5
4
4
4
4
2
1
1
1
1
1
1
1
1
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Immunogen GST-6myc-Tag fusion protein
Sequence EQKLISEEDL
Clone PL14
Isotype IgG1
Specificity This antibody recognizes Myc-Tag peptide sequence (EQKLISEEDL).
Purification Protein-A Sepharose
Alternative Name C-Myc Epitope Tag
Target Type Tag
Background Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VAV-G- Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
Gene ID 4609
NCBI Accession NP_002458
UniProt P01106
Research Area Transcription Factors, Cancer, Cell Cycle
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer NaPB ( pH 8.0), 0.3 M NaCl and 0.09 % NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Background publications Heald, McLoughlin, McKeon: "Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase." in: Cell, Vol. 74, Issue 3, pp. 463-74, 1993 (PubMed).

Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).