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Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (Isoform A)

Details for Product anti-CASP8 Antibody No. ABIN1105681, Supplier: Log in to see
Antigen
Epitope
Isoform A, C-Term
45
17
17
13
10
10
8
8
7
7
6
6
5
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
291
91
64
17
13
4
1
1
1
Host
Rabbit
259
65
11
2
Clonality
Polyclonal
Conjugate
This Caspase 8 antibody is un-conjugated
16
11
6
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
276
111
71
44
39
26
19
16
11
10
8
5
2
2
1
1
1
1
1
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Immunogen A 16 amino acid peptide located near the carboxy-terminus of human caspase-8 isoform A.
Isotype IgG
Specificity This antibody recognises an epitope within the c-terminal region (CT) of caspase-8, otherwise known as Fas-linked ICE-like protease (FLICE), which shares homology with both Fas-associated via death domain (FADD) and the ICE/Ced-3 family of cysteine proteases.
Purification Affinity chromatography
Alternative Name Caspase-8 (CASP8 Antibody Abstract)
Background Caspase-8 is classified as an initiator caspase and acts as the most upstream trigger of Fas-Receptor (CD95) and tumour necrosis factor receptor 1 (TNFR1) mediated apoptosis. Upon activation, the cell death receptor Fas, links with the adaptor molecule FADD via corresponding death domains (DD). FADD in turn then binds via death effector domains (DEDs) to procaspase-8, which then undergoes oligomerization and autocatalytic activation. Active Caspase-8 is capable of cleaving all other known caspases, and also cleaves the Bcl-2 family member BH3-interacting domain death agonist (BID), along with the Bcl-2 associated protein p28Bap31 (2).Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog
Gene ID 841
NCBI Accession NP_001073593
UniProt Q14790
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (Isoform A) (ABIN1105681) anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (C-Term), (Isoform A) antibody
 image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (Isoform A) (ABIN1105681) anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (C-Term), (Isoform A) antibody (Image 2)
Background publications Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999 (PubMed).

Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).