Macrophage Mannose Receptor 1 antibody (Biotin)

Catalog No. ABIN1106049

Product Details anti-Macrophage Mannose Receptor 1 Antibody (Biotin)

Target: Macrophage Mannose Receptor 1 (MRC1) (Mannose Receptor, C Type 1 (MRC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Macrophage Mannose Receptor 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: The monoclonal antibody 15-2 recognizes the mannose receptor (MR), also known as CD206, a member of the vertebrate C-type lectin family. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein G

Immunogen: Purified human mannose receptor from human placental tissue

Clone: 15-02-14

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azid, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Macrophage Mannose Receptor 1

Target: Macrophage Mannose Receptor 1 (MRC1) (Mannose Receptor, C Type 1 (MRC1))

Alternative Name: CD206 / MRC1 (MRC1 Products)

Synonyms: CD206 antibody, CLEC13D antibody, CLEC13DL antibody, MMR antibody, MRC1L1 antibody, bA541I19.1 antibody, AW259686 antibody, MR antibody, mrc1 antibody, MGC86525 antibody, MRC1 antibody, MRC1L-A antibody, mannose receptor C-type 1 antibody, mannose receptor, C type 1 antibody, mannose receptor C-type 1 L homeolog antibody, mediator of replication checkpoint 1 antibody, MRC1 antibody, Mrc1 antibody, mrc1.L antibody, mrc1 antibody

Background: The mannose receptor, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 175 kDa single-pass type I transmembrane receptor consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex.Synonyms: C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, CLEC13D, CLEC13DL, MRC1L1, Macrophage mannose receptor, Macrophage mannose receptor 1-like protein 1

Gene ID: 4360

NCBI Accession: NP_002429

UniProt: P22897