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Antibodies
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anti-alpha-Fetoprotein (AFP) antibody
alpha-Fetoprotein (AFP) antibody
| Antigen |
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| Synonyms |
FETA, HPAFP, AFP, MGC128267 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (192), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (25), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Application
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Alternatives ELISA, Western Blotting (WB), Immunoelectrophoresis (IEP), Immunodiffusion (ID)
Western Blotting (WB) (65), Immunofluorescence (IF) (60), Flow Cytometry (FACS) (56), Enzyme Immunoassay (EIA) (55), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (19), Radioimmunoassay (RIA) (19), Immunoprecipitation (IP) (16), ELISA (Capture) (5), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunodiffusion (ID) (3), Lateral Flow (LF) (3), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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| Catalog no. |
ABIN110610 |
| Quantity |
1mg (Variants) |
| Price |
788.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Immunogen
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Purified human alpha-fetoprotein
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Format
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Affinity-purified
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Description
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IgY(.Fc) to human alpha fetoprotein is developed in duck by using human alpha fetoprotein as immunogen. Affinity purified IgY(. Fc)is purified by alpha fetoprotein coupling chromatography. The product is supplied as a solution in 10mM phosphate buffer, 0.14M sodium chloride, pH 7.2, containing 0.02% NaN 3 as a preservative.
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Specificity
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A single arc was shown by Immunoelectrophoresis while the antibody was reacting with part-pure alpha-fetoprotein and normal human serum. Similar specific reactions were observed when the antibody was tested by western blot assay.
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Application Notes
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Titre: . 1.0 mg/ml, The concentration was determined by 280 nm by spectrophotometer, using 1.4 as the extinction coefficient
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Purity
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Determined by SDS-PAGE( blue stain), showing that the IgY(.Fc) constitute was. 90% of total protein, and the IgY and IgY(.Fc) constitute was . 95% of total protein.
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Storage
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For continuous use, store at 0-5 degrees
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Research Area
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Serum/Plasma Proteins
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Restrictions
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For Research Use only
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Alternatives for antigen "alpha-Fetoprotein (AFP)", type "Antibodies"
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Hosts
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Mouse (128), Rabbit (82), Goat (10), Sheep (3), Duck (1)
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Reactivities
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Human (192), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (25), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Western Blotting (WB) (65), Immunofluorescence (IF) (60), Flow Cytometry (FACS) (56), Enzyme Immunoassay (EIA) (55), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (19), Radioimmunoassay (RIA) (19), Immunoprecipitation (IP) (16), ELISA (Capture) (5), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunodiffusion (ID) (3), Lateral Flow (LF) (3), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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Conjugates
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HRP (7), Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Alexa Fluor 555 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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N-Term (3), a (2), Center (1), b (1), c (1)
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