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Glycophorin A antibody (Glycophorin A (MNS Blood Group)) (FITC)

Details for Product anti-GYPA Antibody No. ABIN1106121, Supplier: Log in to see
Antigen
  • AI853584
  • CD235a
  • gpa
  • GPA
  • GPErik
  • GPSAT
  • GYPA
  • HGpMiV
  • HGpMiXI
  • HGpSta(C)
  • MN
  • MNS
  • PAS-2
Reactivity
Human
290
21
15
4
Host
Mouse
173
68
35
19
Clonality (Clone)
Monoclonal ()
Conjugate
This Glycophorin A antibody is conjugated to FITC
49
40
14
9
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
180
83
55
49
42
33
24
11
10
10
10
5
5
5
3
3
2
2
1
Supplier
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Clone NaM10-6G4
Isotype IgG2a
Specificity Clone NAM10-6G4 produces mouse IgG2a immunoglobulins directed against the human CD235a antigen (41 kDa) which is expressed as a cell surface sialoglycoprotein. This antibody may be effectively used for differentiation in acute leukemias, e.g. presence of the GpA antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells. Using the CD235a antibody, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias [3,4]. CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19 [6].
Purification Affinity chromatography
Alternative Name CD235a / GYPA (GYPA Antibody Abstract)
Background Glycophorin A (GpA) antigen (CD235a) is exclusively expressed on human erythroid cells and their progenitors and not on lymphoid or granulocytic progenitor cells and therefore is a very useful marker for detection of the erythroid cell lineage [1,2]. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from (pro-)erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha
Gene ID 2993
NCBI Accession NP_002090
UniProt P02724
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.
Supplier Images
 image for anti-Glycophorin A antibody (Glycophorin A (MNS Blood Group))  (FITC) (ABIN1106121) anti-Glycophorin A (MNS Blood Group) (GYPA) antibody (FITC)
Background publications Cuneo, Van Orshoven, Michaux, Boogaerts, Louwagie, Doyen, Dal Cin, Fagioli, Castoldi, Van den Berghe: "Morphologic, immunologic and cytogenetic studies in erythroleukaemia: evidence for multilineage involvement and identification of two distinct cytogenetic-clinicopathological types." in: British journal of haematology, Vol. 75, Issue 3, pp. 346-54, 1990

Loken, Shah, Dattilio, Civin: "Flow cytometric analysis of human bone marrow: I. Normal erythroid development." in: Blood, Vol. 69, Issue 1, pp. 255-63, 1987

Villeval, Cramer, Lemoine, Henri, Bettaieb, Bernaudin, Beuzard, Berger, Flandrin, Breton-Gorius: "Phenotype of early erythroblastic leukemias." in: Blood, Vol. 68, Issue 5, pp. 1167-74, 1986

Liszka, Majdic, Bettelheim, Knapp: "Glycophorin A expression in malignant hematopoiesis." in: American journal of hematology, Vol. 15, Issue 3, pp. 219-26, 1983

Andersson, Gahmberg, Teerenhovi, Vuopio: "Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia." in: International journal of cancer. Journal international du cancer, Vol. 24, Issue 6, pp. 717-20, 1980