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CD235a/GYPA antibody (FITC)

GYPA Reactivity: Human FACS Host: Mouse Monoclonal NaM10-6G4 FITC
Catalog No. ABIN1106121
  • Target See all CD235a/GYPA (GYPA) Antibodies
    CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
    Reactivity
    • 131
    • 17
    • 12
    • 10
    Human
    Host
    • 69
    • 64
    • 14
    • 1
    Mouse
    Clonality
    • 90
    • 56
    Monoclonal
    Conjugate
    • 58
    • 14
    • 12
    • 11
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This CD235a/GYPA antibody is conjugated to FITC
    Application
    • 103
    • 57
    • 42
    • 30
    • 26
    • 24
    • 15
    • 13
    • 13
    • 13
    • 13
    • 5
    • 5
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    • 4
    • 3
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    Clone NAM10-6G4 produces mouse IgG2a immunoglobulins directed against the human CD235a antigen (41 kDa) which is expressed as a cell surface sialoglycoprotein. This antibody may be effectively used for differentiation in acute leukemias, e.g. presence of the GpA antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells. Using the CD235a antibody, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias [3,4]. CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19 [6].
    Purification
    Affinity chromatography
    Clone
    NaM10-6G4
    Isotype
    IgG2a
    Top Product
    Discover our top product GYPA Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.
  • Target
    CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
    Alternative Name
    CD235a / GYPA (GYPA Products)
    Synonyms
    GYPA antibody, gpa antibody, AI853584 antibody, CD235a antibody, GPA antibody, GPErik antibody, GPSAT antibody, HGpMiV antibody, HGpMiXI antibody, HGpSta(C) antibody, MN antibody, MNS antibody, PAS-2 antibody, glycophorin A (MNS blood group) antibody, glycophorin A antibody, GYPA antibody, Gypa antibody
    Background
    Glycophorin A (GpA) antigen (CD235a) is exclusively expressed on human erythroid cells and their progenitors and not on lymphoid or granulocytic progenitor cells and therefore is a very useful marker for detection of the erythroid cell lineage [1,2]. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from (pro-)erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha
    Gene ID
    2993
    NCBI Accession
    NP_002090
    UniProt
    P02724
    Pathways
    Maintenance of Protein Location
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