Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TLR9 antibody (Extracellular Domain, N-Term) (Biotin)

TLR9 Reactivity: Human, Mouse, Dog WB, IHC (p), IF, IHC (fro), EIA Host: Mouse Monoclonal 5G5 Biotin
Catalog No. ABIN1106225
  • Target See all TLR9 Antibodies
    TLR9 (Toll-Like Receptor 9 (TLR9))
    Binding Specificity
    • 18
    • 15
    • 8
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-815, Extracellular Domain, N-Term
    Reactivity
    • 93
    • 38
    • 13
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Dog
    Host
    • 64
    • 32
    • 7
    • 4
    • 1
    Mouse
    Clonality
    • 72
    • 36
    Monoclonal
    Conjugate
    • 62
    • 13
    • 11
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TLR9 antibody is conjugated to Biotin
    Application
    • 76
    • 49
    • 45
    • 45
    • 22
    • 14
    • 12
    • 10
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, mouse, canine
    Purification
    Protein G
    Immunogen
    Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
    Clone
    5G5
    Isotype
    IgG2a
    Top Product
    Discover our top product TLR9 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, 0.1 % bovine serum albumin, 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2 - 8 °C.
  • Target
    TLR9 (Toll-Like Receptor 9 (TLR9))
    Alternative Name
    CD289 / TLR9 (TLR9 Products)
    Synonyms
    CD289 antibody, toll like receptor 9 antibody, toll-like receptor 9 antibody, TLR9 antibody, Tlr9 antibody
    Background
    Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens. Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.Synonyms: Toll-like receptor 9, UNQ5798/PRO19605
    UniProt
    Q9EQU3
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
You are here:
Support