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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product anti-PTPRC Antibody No. ABIN1106400, Supplier: Log in to see
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • ptprc
  • Ptprc
Reactivity
Human
1377
716
207
137
62
51
45
39
24
21
18
8
5
5
4
3
3
2
1
1
1
1
1
1
Host
Mouse
1439
783
226
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is un-conjugated
288
237
177
98
61
59
56
48
44
38
35
35
33
32
32
32
27
25
25
24
21
17
14
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12
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9
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5
5
5
5
5
5
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4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
2005
522
503
502
484
429
420
382
202
46
40
38
36
26
16
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
2
1
1
Options
Supplier
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Clone MB1
Isotype IgG1
Specificity Clone MB1 (anti-CD45RA) detects a restricted epitope (CD45RA) of the CD45 complex. MB1 recognizes normal and neoplastic B cells but not mature plasma cells, monocytes, granulocytes and 50 % of mature T cells. MB1 does not react with non-lymphoid cell types. MB1 reacts with lymphocytes in B cell areas of normal lymphoid tissues. Other isoforms can be detected using anti-CD45RB (clone MT4, cat.no. AM39021), or anti-CD45RC (clone MT2, cat.no. AM39022). Detection of the different isoforms can distinguish, for example, between naive T cells and memory T cells, which is of interest in patients with immunodeficiency and autoimmune diseases.
Purification Affinity chromatography
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background The CD45 Molecule is also known as the Leukocyte Common Antigen (LCA) or T200 antigen, and is comprised of different glycoproteins ranging from 180-240 kD [1,2]. CD45 is a family of transmembrane protein tyrosine phosphatases critically involved in the regulation of lymphocyte activation signals. Expression of CD45 is found on all hemopoietic cells, e.g. granulocytes, monocytes, macrophages and lymphocytes, except mature erythroid cells. In humans, there is heterogeneous expression of CD45 isoforms (RA, RB, RO, RC) on lymphocyte subpopulations. Detection of the different isoforms can distinguish, for example, between naive T cells and memory T cells, which is of interest in patients with immunodeficiency and autoimmune diseases.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 5788
NCBI Accession NP_002829
UniProt P08575
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.
Supplier Images
 image for anti-CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (ABIN1106400) anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
Background publications Young, Ramage, Gaston, Beverley: "In vitro responses of human CD45R0brightRA- and CD45R0-RAbright T cell subsets and their relationship to memory and naive T cells." in: European journal of immunology, Vol. 27, Issue 9, pp. 2383-90, 1997 (PubMed).

Thomas: "The leukocyte common antigen family." in: Annual review of immunology, Vol. 7, pp. 339-69, 1989 (PubMed).

Streuli, Hall, Saga, Schlossman, Saito: "Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens." in: The Journal of experimental medicine, Vol. 166, Issue 5, pp. 1548-66, 1987 (PubMed).

Hall, DArdenne, Butler, Habeshaw, Stansfeld: "New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections." in: Journal of clinical pathology, Vol. 40, Issue 2, pp. 151-6, 1987 (PubMed).

Poppema, Hollema, Visser, Vos: "Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections." in: The American journal of pathology, Vol. 127, Issue 3, pp. 418-29, 1987 (PubMed).

West, Warford, Fray, Allen, Campbell, Lauder: "The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections." in: The Journal of pathology, Vol. 150, Issue 2, pp. 89-101, 1987 (PubMed).