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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (PE)

Details for Product anti-PTPRC Antibody No. ABIN1106410, Supplier: Log in to see
Antigen
  • B220
  • CD45
  • Cd45
  • cd45
  • CD45R
  • GP180
  • L-CA
  • Lca
  • LCA
  • loc
  • Ly-5
  • LY5
  • Lyt-4
  • PTPRC
  • RT7
  • si:dkeyp-47c5.1
  • T200
Reactivity
Human
1201
727
207
135
62
51
41
39
23
21
17
8
5
5
4
3
3
2
1
1
1
1
1
1
Host
Mouse
1278
782
217
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is conjugated to PE
280
227
168
98
53
52
49
40
35
35
30
27
27
25
24
24
24
24
22
17
17
17
14
14
13
13
10
10
9
7
6
6
6
6
6
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
1852
525
499
472
429
373
324
227
191
50
46
40
26
16
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
2
1
1
Supplier
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Clone MT4
Isotype IgG1
Specificity Clone MT4 reacts with the 190, 205 and 220 kDa isoforms of the cell-surface antigen CD45RB. Variations in CD45RB expression can discriminate between Th1 and Th 2 cells, i.e. CD45RBbright and CD45RB-dim respectively [8]. CD45RB bright expression on T cells correlates with higher proliferation and IFN-g production in comparison to CD45RB dim expression. 90 % of lymphocytes are CD45RB positive. Other isoforms can be detected using anti-CD45RA (clone MB1, cat.no. AM39020), or anti-CD45RC (clone MT2, cat.no. AM39022).
Purification Affinity chromatography
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background The CD45 Molecule is also known as the Leukocyte Common Antigen (LCA) or T200 antigen, and is comprised of different glycoproteins ranging from 180-240 kD [1,2]. CD45 is a family of transmembrane protein tyrosine phosphatases critically involved in the regulation of lymphocyte activation signals. Expression of CD45 is found on all hemopoietic cells, e.g. granulocytes, monocytes, macrophages and lymphocytes, except mature erythroid cells. In humans, there is heterogeneous expression of CD45 isoforms (RA, RB, RO, RC) on lymphocyte subpopulations. Detection of the different isoforms can distinguish, for example, between naive T cells and memory T cells, which is of interest in patients with immunodeficiency and autoimmune diseases.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 5788
NCBI Accession NP_002829
UniProt P08575
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.
Supplier Images
 image for anti-CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)  (PE) (ABIN1106410) anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (PE)
Background publications Young, Ramage, Gaston, Beverley: "In vitro responses of human CD45R0brightRA- and CD45R0-RAbright T cell subsets and their relationship to memory and naive T cells." in: European journal of immunology, Vol. 27, Issue 9, pp. 2383-90, 1997 (PubMed).

Thomas: "The leukocyte common antigen family." in: Annual review of immunology, Vol. 7, pp. 339-69, 1989 (PubMed).

Streuli, Hall, Saga, Schlossman, Saito: "Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens." in: The Journal of experimental medicine, Vol. 166, Issue 5, pp. 1548-66, 1987 (PubMed).

Hall, DArdenne, Butler, Habeshaw, Stansfeld: "New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections." in: Journal of clinical pathology, Vol. 40, Issue 2, pp. 151-6, 1987 (PubMed).

Poppema, Hollema, Visser, Vos: "Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections." in: The American journal of pathology, Vol. 127, Issue 3, pp. 418-29, 1987 (PubMed).

West, Warford, Fray, Allen, Campbell, Lauder: "The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections." in: The Journal of pathology, Vol. 150, Issue 2, pp. 89-101, 1987 (PubMed).