CD55 antibody (PE)

Catalog No. ABIN1106466

Product Details anti-CD55 Antibody (PE)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: The antibody detects human CD55. Other species not tested. Cellular expression: platelets, monocytes, lymphocytes and erythrocytes.

Purification: Affinity chromatography

Clone: NaM16-4D3

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Fluorochrome labelled product is photosensitive and should be protected from light.

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 / DAF (CD55 Products)

Synonyms: CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody

Background: CD55 is expressed as a 60-70 kD single chain Glycosylphosphatidylinositol (GPI) anchored glycoprotein (in erythrocytes). In other cells the molecular weight is somewhat larger. It is comprised of four N-terminal short consensus repeats (SCR) modules (4 Cys in 1-3,2-4 linkage) with C3b/C4b binding and regulator activity in SCR 2,3,4. CD55 is often involved in the protection of cells from autologous compliment-mediated injury and are partially or completely lacking in peripheral blood cells. CD55 inhibits the formation and accelerates the decay of C3/C5 convertase complexes both of classical and alternative pathway [1]. CD55 sets a protective barrier threshold against inappropriate complement activation and deposition on plasma membranes, especially by the classical pathway of complement activation, by limiting formation and half-life of the C3 convertases. Since CD55 is well known membrane-associated complement regulator protein, like CD59, and present on all blood cells, anti-CD55 and anti-CD59 appear to be the most effective Mabs to detect very minor negative cell subsets (less than 1 % on erythrocytes or less than 5 % on PMN leukocytes.Synonyms: Complement decay-accelerating factor

Gene ID: 1604

NCBI Accession: NP_000565

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity