FLIP antibody (C-Term, long form)

Catalog No. ABIN1106673

Product Details anti-FLIP Antibody

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Binding Specificity: C-Term, long form

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes FLIP-L No cross reactivity with other proteins.

Purification: Immunoaffinity Chromatography

Immunogen: A synthetic peptide corresponding to a sequence mapping at the C-terminal of Human FLIP- L, identical to the related Mouse sequence.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.2 mL of distilled water to yield a concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg Sodium Azide

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody after reconstitution at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR / Casper / I-FLICE (CFLAR Products)

Synonyms: Clarp1 antibody, cflar antibody, CFLAR antibody, FLIP antibody, CASH antibody, CASP8AP1 antibody, CLARP antibody, Casper antibody, FLAME antibody, FLAME-1 antibody, FLAME1 antibody, I-FLICE antibody, MRIT antibody, c-FLIP antibody, c-FLIPL antibody, c-FLIPR antibody, c-FLIPS antibody, 2310024N18Rik antibody, A430105C05Rik antibody, Cash antibody, ENSMUSG00000072980 antibody, Flip antibody, Gm9845 antibody, c-Flip antibody, CASP8 and FADD-like apoptosis regulator a antibody, CASP8 and FADD like apoptosis regulator antibody, CASP8 and FADD like apoptosis regulator S homeolog antibody, CASP8 and FADD-like apoptosis regulator antibody, cflara antibody, CFLAR antibody, cflar antibody, cflar.S antibody, Cflar antibody

Background: Irmler et al. (1997) isolated human cDNAs encoding 2 isoforms of FLIP. FLIP(L), the longer isoform, contains 2 DEDs and a caspase-like domain, and FLIP(S), the shorter isoform, contains only the 2 DEDs followed by a C-terminal extension of approximately 50 amino acids. High levels of FLIP(L) protein were detected in melanoma cell lines and malignant melanoma tumors. FLIP may be implicated in tissue homeostasis as an important regulator of apoptosis.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP

Gene ID: 8837

NCBI Accession: NP_001120655

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development