Collagen, Type I (COL1) antibody

Details for Product No. ABIN1106738
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(142), (72), (63), (41), (25), (21), (14), (9), (8), (6), (6), (4), (2), (2), (2), (2), (2), (1), (1)
(173), (30), (25), (2), (1)
Clonality (Clone)
Monoclonal ()
(23), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA
(179), (115), (106), (94), (65), (60), (56), (26), (20), (19), (18), (3), (3), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN1106738
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Immunogen Human type I collagen (Sigma-Aldrich, C5483), digested with Collagenase from Clostridium histolyticum (Sigma-Aldrich, C0773)
Clone 2G8
Isotype IgM
Specificity This antibody, compared to collagen type 1, preferentially recognizes Collagenase-digested Human type I Collagen. There is some cross reactivity with Human epidermal keratinocytes. No cross reactivity has been detected with Mouse Collagen. In immunoblotting (not reducing conditions), the antibody reacts with both intact as digested collagen type 1. The antibody doesn’t cross react with Human type IV Collagen.
Purification Ammonium Sulphate precipitation and DEAE Chromatography
Alternative Name Collagen Type I
Background Collagens play a dominant role in maintaining the structural integrity of organs and tissues (1), but they also regulate cell polarity, migration, survival and phenotype, and provide diffusible signaling molecules following breakdown (2). Within the family of collagens that currently comprises 21 members, type I collagen is included in the group of fibril-forming collagens based on its structural and functional features. Type I collagen is ubiquitous in all vertebrates and is among the largest and most complex of all macromolecules. It accounts for 80-90% of all collagenous proteins in skin (3). The helical trimeric molecules of type I collagen compromise two alpha1(I) chains and one alpha2(I) chain, encoded by two separate genes Col(I)alpha1 and Col(I)alpha2, respectively (3). Matrix metalloproteinase (MMP)-1,-8, -13 and -14 cleave the peptide bonds between residues Gly775 and Ile776 of the alpha1(I) chain and Gly775 and Leu776 of the alpha2(I) chains, resulting in ¼ and ¾ fragments.
Alternate names: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
Gene ID 1277
NCBI Accession NP_000079
UniProt P02452
Research Area Extracellular Matrix
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer 100 mM Tris-HCl, pH 8.0 0.05% Sodium Azide 50% (v/v) Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in small aliquots) at -20°C. Avoid freeze-thaw cycles.
Expiry Date 12 months
Background publications Vuorio, de Crombrugghe: "The family of collagen genes." in: Annual review of biochemistry, Vol. 59, pp. 837-72, 1990 (PubMed).

Egeblad, Werb: "New functions for the matrix metalloproteinases in cancer progression." in: Nature reviews. Cancer, Vol. 2, Issue 3, pp. 161-74, 2002 (PubMed).

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