Collagen Type I antibody

Details for Product No. ABIN1106738
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Antigen
Reactivity
Human
(32), (14), (9), (8), (8), (7), (5), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host
Mouse
(45), (10), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blotting (WB)
(42), (38), (37), (29), (28), (21), (13), (10), (7), (6), (3), (3), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN1106738
940.50 $
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Immunogen Human type I collagen (Sigma-Aldrich, C5483), digested with Collagenase from Clostridium histolyticum (Sigma-Aldrich, C0773)
Clone 2G8
Isotype IgM
Specificity This antibody, compared to collagen type 1, preferentially recognizes Collagenase-digested Human type I Collagen. There is some cross reactivity with Human epidermal keratinocytes. No cross reactivity has been detected with Mouse Collagen. In immunoblotting (not reducing conditions), the antibody reacts with both intact as digested collagen type 1. The antibody doesn't cross react with Human type IV Collagen.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ammonium Sulphate precipitation and DEAE Chromatography
Background Collagens play a dominant role in maintaining the structural integrity of organs and tissues (1), but they also regulate cell polarity, migration, survival and phenotype, and provide diffusible signaling molecules following breakdown (2). Within the family of collagens that currently comprises 21 members, type I collagen is included in the group of fibril-forming collagens based on its structural and functional features. Type I collagen is ubiquitous in all vertebrates and is among the largest and most complex of all macromolecules. It accounts for 80-90 % of all collagenous proteins in skin (3). The helical trimeric molecules of type I collagen compromise two α1(I) chains and one α2(I) chain, encoded by two separate genes Col(I)α1 and Col(I)α2, respectively (3). Matrix metalloproteinase (MMP)-1,-8, -13 and -14 cleave the peptide bonds between residues Gly775 and Ile776 of the α1(I) chain and Gly775 and Leu776 of the α2(I) chains, resulting in 1/4 and 3/4 fragments.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
Gene ID 1277
NCBI Accession NP_000079
UniProt P02452
Research Area Extracellular Matrix
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 2 mg/mL
Buffer 100 mM Tris-HCl, pH 8.0, 0.05 % Sodium Azide, 50 % (v/v) Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in small aliquots) at -20 °C. Avoid freeze-thaw cycles.
Background publications Vuorio, de Crombrugghe: "The family of collagen genes." in: Annual review of biochemistry, Vol. 59, pp. 837-72, 1990 (PubMed).

Egeblad, Werb: "New functions for the matrix metalloproteinases in cancer progression." in: Nature reviews. Cancer, Vol. 2, Issue 3, pp. 161-74, 2002 (PubMed).

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