CR1L antibody (FITC)

Catalog No. ABIN1106830

Product Details anti-CR1L Antibody (FITC)

Target: CR1L (Complement Component (3b/4b) Receptor 1-Like (CR1L))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CR1L antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with rat-complement regulator Crry.

Cross-Reactivity (Details): Species reactivity (tested):Rat

Purification: Potein G

Immunogen: Rat microglial cells

Clone: TLD-1C11

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for CR1L

Target: CR1L (Complement Component (3b/4b) Receptor 1-Like (CR1L))

Alternative Name: CR1L / CRRY (CR1L Products)

Synonyms: CREG antibody, Cr1 antibody, Crry antibody, Mcp antibody, mCRY antibody, complement C3b/C4b receptor 1 like antibody, complement component (3b/4b) receptor 1-like antibody, CR1L antibody, Cr1l antibody

Background: Complement comprises a system of soluble serum proteins that, upon activiation by antibody (classical pathway), sugars (lectin pathway), or bacterial and foreign surfaces (alternative pathway), directly causes cell damage. Complement is constantly activated at low levels in serum, requiring cells to be protect themselves from damage. In human, cells are protected from complement-mediated damage by expression of membrane-bound complement regulatory proteins, which act at serveral points along the cascade. Human cells are protected by decay-accelerating factor (DAF, CD55), membrane cofactor protein (MCP, CD46) and membrane attack complex inhibitor protein 1 (MIN1, CD59). Furthermore the rat analogues of DAF, MCP and CD59 have been characterized. Rats and mice express a unique membrane complement regulator of the activating pathways, called complement receptor type-1 related gene Y (Crry). In rats there are two isoforms of Crry, with six or seven short consensus repeats (SCR), encoding 65-70 and 75-85 kDa proteins, respectively, which are both transmembrane anchored. Although Crry has the functional properties of both DAF and MCP, it does not replace them. All rat blood cells express Crry whereas approximately 37 and 39 % of the rat T cells express respectively DAF and CD59. Studies in rat revealed that despite lacking DAF and CD59, these double-negative rat T cells were not more susceptible to homologous complement lysis than cells that expressed both DAF and CD59. However, blocking of the only other known rat complement regulator, Crry, enhanced lysis only at the double-negative cells. In rats, Crry exerts powerful control in the activation pathways where it acts both as a cofactor and as a decay accelerator to inactivate convertases. Crry has been succesfully expressed and refolded in bacteria. The refolded protein has full-complement regulatory activity in vitro, and has been applied in rat models of arthritis and other inflammatory diseases.Synonyms: Antigen 5I2, Complement component receptor 1-like protein, Complement regulatory protein Crry

Gene ID: 54243

NCBI Accession: NP_001005265

UniProt: Q63135

Pathways: Complement System