Basal Cell Cytokeratin antibody
Reactivity: Human, Rabbit, Rat, Guinea Pig, Chicken, Dog, Hamster, Pig, Quail, Zebrafish (Danio rerio)
WB, FACS, IHC (fro), IF
Host: Mouse
Monoclonal
RCK103
unconjugated
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- Target
- Basal Cell Cytokeratin
- Reactivity
- Human, Rabbit, Rat, Guinea Pig, Chicken, Dog, Hamster, Pig, Quail, Zebrafish (Danio rerio)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- Specificity
- Clone RCK103 is a Cytokeratin antibody recognizing (amongst others) Cytokeratin 5. This monoclonal antibody stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
- Cross-Reactivity (Details)
- Species reactivity (tested):Canine, Chicken, Guinea Pig, Hamster, Human, Quail, Rabbit, Rat, Swine, Zebrafish.
- Purification
- Purified
- Immunogen
- A mix of cell preparations containing Human cytokeratins.
- Clone
- RCK103
- Isotype
- IgG1
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anti-Basal Cell Cytokeratin antibody
Reactivity: Human, Rabbit, Rat, Guinea Pig, Chicken, Dog, Hamster, Pig, Quail, Zebrafish (Danio rerio) WB, IHC, FACS, IHC (fro), ICC Host: Mouse Monoclonal RCK103 unconjugated
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted (protected from light) at 2-8 °C Do Not Freeze!
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- Target
- Basal Cell Cytokeratin
- Alternative Name
- Cytokeratin (Basal Cell)
- Background
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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