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Keratin 8 (KRT8) antibody

Details for Product No. ABIN1106942, Supplier: Log in to see
Antigen
  • ck8
  • cyk8
  • k2c8
  • card2
  • krt2-5
  • MGC69490
  • KRT8
  • DKFZp468F2127
  • CARD2
  • CK-8
  • CK8
  • CYK8
  • K2C8
  • K8
  • KO
  • KRT2-8
  • AA960620
  • AL022697
  • AU019895
  • Card2
  • EndoA
  • Krt-2.8
  • Krt2-8
  • CYKER
  • DreK8
  • cb186
  • krt2-8
  • sb:cb186
  • wu:fa20h05
  • wu:fa95h10
  • wu:fb96c06
  • zf-K8
  • zfk8
  • KERATIN8
Reactivity
Human
455
103
95
24
24
23
18
17
7
5
5
4
4
2
2
1
1
1
1
Host
Mouse
248
221
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
21
15
6
4
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
299
194
143
123
118
69
63
62
44
26
14
11
4
2
2
2
1
1
Supplier
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Immunogen Cytokeratin purified from the Human breast carcinoma cell line MC-7
Clone M20
Isotype IgG1
Specificity This Monoclonal Antibody Clone M20 does stain almost all epithelial cells. In immunoblotting experiments the antibody recognizes only Keratin 8 (52,2 kDa) and does not cross react with other cytokeratins. Frozen sections give excellent results in immunoperoxidase and immunofluorescence tests. Cross reactivity: Vimentin : not detectable Desmin : not detectable GFAP : not detectable Neurofilament : not detectable.Frozen sections give excellent results in immunoperoxidase and immunofluorescence tests.Cross reactivity:
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Supernatant
Alternative Name Cytokeratin 8 (KRT8 Antibody Abstract)
Background Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
Gene ID 3856
NCBI Accession NP_002264
UniProt P05787
Research Area Cancer, Cytoskeleton, Cytokeratins, Extracellular Matrix
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 20 μg antibody/ml
Buffer 10 mM Sodium Azide, 1 % FCS
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or mix with an equal volume of glycerol and store (in aliquots) at -20 °C for longer.
Background publications Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987 (PubMed).

Odin, Obrink: "Quantitative determination of the organ distribution of the cell adhesion molecule cell-CAM 105 by radioimmunoassay." in: Experimental cell research, Vol. 171, Issue 1, pp. 1-15, 1987 (PubMed).

Kasper, Stosiek, van Muijen et al.: "Cell type heterogeneity of intermediate filament expression in epithelia of the human pituitary gland." in: Histochemistry, Vol. 93, Issue 1, pp. 93-103, 1990 (PubMed).

van Niekerk, Boerman, Ramaekers et al.: "Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas." in: The American journal of pathology, Vol. 138, Issue 2, pp. 455-63, 1991 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).