Proopiomelanocortin (POMC) (AA 1-24) antibody

Antigen: Proopiomelanocortin (POMC)

Synonyms: MSH, ACTH, pomc, SI:bZ36D5.1, SI:bZ36D5.4, si:bz36d5.2, POMC, LPH, NPP, POC, CLIP

Epitope: AA 1-24

Clonality: Monoclonal (57 (BGN-1388-66))

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN110701

Additional Information

Characteristics: Synonyms: Corticotropin, Adrenocorticotropic hormone, ACTH

Alternative name: Adrenocorticotropic Hormone (ACTH) (1-24)

Swiss-Prot: P01189

Immunogen: Synthetic peptide corresponding to the amino acids 1-24 of the Human ACTH conjugated toKeyhole Limpet Haemocyanin. AA Sequence: SYSMEHFRWGKPVGKKRRPVKVYP

Isotype: IgG1  (Matching secondary antibodies)

Clone: 57 (BGN-1388-66)

Description: ACTH, released from the anterior pituitary gland in response to corticotropin-releasinghormone from the hypothalamus, acts on the adrenal cortex to stimulate the production ofcorticosteroids such as cortisol, involved in the response to stress. Studies have shownthat the administration of 1-24 ACTH increases blood pressure, believed to be attributed toan increase in ACTH-stimulated cortisol secretion, in association with increased cardiacoutput.

Specificity: This antibody recognises 1-24 ACTH (Synacthen) which, as a synthetic analogue ofnaturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserveand for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation. It does not recognise 17-39 ACTH (CLIP). Species: Human. Cross-reacts with Rat N-terminal ACTH. Others not tested.

Application Details

Application Notes: ELISA. Immunohistochemistry on Formalin Fixed Paraffin Embedded Sections: 1/100-1/200(Requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH 7.2, containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at -20°C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only