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GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN1107350, Supplier: Login to see
Antigen
Reactivity
Aequorea victoria
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2
1
1
Host
Mouse
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157
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38
11
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
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28
20
15
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3
3
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3
3
2
1
1
1
1
1
1
1
1
1
1
1
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1
1
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1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
412
235
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27
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15
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8
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3
2
1
1
1
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1
Supplier
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Immunogen This monoclonal antibody is produced by immunizing animals with fulllength recombinant GFP.
Clone 7G9
Isotype IgG1
Purification Affinity Purified
Alternative Name GFP
Target Type Tag
Background The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. When expressed in cells, fluorescence is produced without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. A number of GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wild type GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. Thus, GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.Synonyms: GFP-Tag, Green fluorescent protein
Gene ID 6100
UniProt P42212
Research Area Tags/Labels
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN1107350) anti-Green Fluorescent Protein (GFP) antibody
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN1107350) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
Background publications Chalfie, Tu, Euskirchen et al.: "Green fluorescent protein as a marker for gene expression." in: Science (New York, N.Y.), Vol. 263, Issue 5148, pp. 802-5, 1994 (PubMed).

Prasher, Eckenrode, Ward et al.: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).