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Details for Product No. ABIN1107476

HA-Tag antibody

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Antigen
Reactivity
»Alternatives Mammalian
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN1107476
Quantity 50 µg
Price
275.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen A synthetic peptide from influenza hemagglutinin epitope coupled to KLH
Sequence YPYDVPDYA
Isotype IgG
Specificity HA-Tag polyclonal antibody detects HA-tagged proteins overexpressed in cells. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. Species Reactivity: Tested: Mammalia
Purification Affinity chromatography
Alternative Name HA Epitope Tag (YPYDVPDYA)
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in acterial, insect and mammalian hosts are in common usage, such expression ectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the urification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA).
Alternate names: HA Tag, HA-Tag, Hemagglutinin Tag
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4,with 0.05% sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

Alternatives for antigen "HA-Tag", type "Antibodies"
Hosts (126), (115), (45), (25), (1), (1)
Reactivities (258), (21), (19), (17), (6), (6), (2), (1), (1), (1)
Applications (243), (155), (104), (65), (50), (41), (30), (20), (13), (12), (9), (6), (6), (4), (4), (3), (2), (2), (2), (1)
Conjugates (30), (20), (18), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Epitopes (10), (2), (1)
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