HRH3 antibody (C-Term)

Catalog No. ABIN1107535

Product Details anti-HRH3 Antibody

Target: HRH3 (Histamine Receptor H3 (HRH3))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Histamine H3 receptor (C-term). No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human HRH3 .Remarks: Sequence is different to the related rat sequence by two amino acides.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for HRH3

Target: HRH3 (Histamine Receptor H3 (HRH3))

Alternative Name: Histamine H3 Receptor (HRH3 Products)

Synonyms: GPCR97 antibody, HH3R antibody, AW049250 antibody, H3R antibody, histamine receptor H3 antibody, HRH3 antibody, Hrh3 antibody

Background: The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems1. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22 % and 21.4 % amino acid sequence homology with the H1 (HRH1) and H2 (HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V,and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.Synonyms: G-protein coupled receptor 97, GPCR97, HH3R

Gene ID: 11255

NCBI Accession: NP_009163

UniProt: Q9Y5N1

Pathways: cAMP Metabolic Process, Feeding Behaviour